1. I don’t recognize you and I know just about everyone in this town. You must not be from this neck of the woods.
2. Excuse me. Can you give me directions? I’m not familiar with this neck of the woods.

The expression is often used to describe what part of the country a person comes from, but it can also be used to describe unfamiliarity with a part of a city or state. It is frequently used in a negative structure.